Learning objectives:

• Describe the principles of flow cytometry, including the components of a flow cytometric evaluation, the characteristics of fluorochromes, the parameters collected, data display and analysis.
• List the immunophenotype of normal hematopoietic and lymphoid cells, including B-cells, T-cells, plasma cells, monocytes, and maturing myeloid cells.
• Describe immunophenotypic abnormalities associated with neoplasms of hematopoietic and lymphoid cells, and how they can be used to diagnose disease and detect minimal residual disease following therapy.
• List the immunophenotype associated with neoplasms of mature B- and T-cells, including chronic lymphocytic leukemia, hairy cell leukemia, high-grade B-cell lymphomas, lymphoma with plasmablastic differentiation, plasma cell myeloma, T-cell large granular lymphocytic leukemia, T-cell prolymphocytic leukemia, Sezary syndrome, angioimmunoblastic T-cells lymphoma and anaplastic large cell lymphoma.
• Discuss the role of flow cytometry in the evaluation for hematopoietic neoplasms, B- and T-lymphoblastic leukemia, acute myeloid leukemia, acute undifferentiated leukemia, mixed phenotype leukemia, blastic plasmacytoid dendritic cell neoplasm, myelodysplastic syndromes and myeloproliferative neoplasms.