Learning objectives:

• Recall the major steps involved in nucleic acid isolation
• Discuss overcoming issues with difficult sample types during nucleic acid isolation
• Detail different reagents and approaches involved with cell lysis and nucleic acid separation
• Compare similarities and differences between DNA and RNA extraction
• Describe different types of liquid phase nucleic acid extraction
• Recall details about the different methods for solid phase nucleic acid extraction
• Discuss measurement and optimization of nucleic acid quantity and quality