Laboratory Genomics

NUCLEIC ACID ISOLATION (ADVANCED)

Learning Lab Advanced

Author information
Linnea Baudhuin, PhD, FACMG
Dr Baudhuin disclosed no conflict of interest

Learning objectives:

  • Recall the major steps involved in nucleic acid isolation
  • Discuss overcoming issues with difficult sample types during nucleic acid isolation
  • Detail different reagents and approaches involved with cell lysis and nucleic acid separation
  • Compare similarities and differences between DNA and RNA extraction
  • Describe different types of liquid phase nucleic acid extraction
  • Recall details about the different methods for solid phase nucleic acid extraction
  • Discuss measurement and optimization of nucleic acid quantity and quality
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